Instrumentation

Below are details about the instrumentation within the ∆Translational Services Recharge Center. Visit the Rates Page for Instrumentation and Services fee schedules.

BioPlex® 200 multiplex assay system (Luminex)

The BioPlex 200 system is a suspension array method which allows interrogation of up to 100 biomolecules (protein, nucleic acid) in a single suspension sample.

nanoString nCounter® MAX Analysis System

NanoString nCounter® MAX is a high throughput direct detection method for RNA, DNA, miRNA and protein detection using direct detection hybridization.

Features:

For use with RNA, DNA, miRNA, and protein analytes; High precision and sensitivity (<1 copy/cell)
Counts native expression via direct hybridization, barcoding and imaging (nCounter® technology)
Rapid results and efficient sample workflow and intuitive data analysis & up to 800 targets/sample tube
Compatible with a wide range of sample input (FFPE, serum, plasma, PMCS, FACS etc.)
Avoids RT and amplification bias potentially incurred using traditional RNA → cDNA methods
Combine RNA, DNA, and protein panels (3D Biology™)

QuantStudio 6 Flex Real-Time PCR

The Applied Biosystems QuantStudio 6 Flex Real-Time PCR System offers a high throughput 96-well 5 color format with intuitive software and easy touch-screen setup. Detects as small as 1.5-fold changes in singleplex reactions and 10 logs dynamic range. Compatible with: Applied Biosystems™ SYBR™, TaqMan

For use in studies interrogating:

Gene expression
miRNA profiling
SNP genotyping
Copy number variation
Protein thermal shift
High resolution melt
Pathogen detection

Flow Cytometry

BD FACScan™

The FACScan™ system is an automated flow cytometer that analyzes cells as they pass through a focused laser beam one at a time in a moving fluid stream. A BD FACScan flow cytometer can be used for routine research applications, immunophenotyping, and DNA cell-cycle analysis. Laser lines: 488, 530, 575 or 650 nm

BD Accuri™ C6